Abstract

Superovulation plays an important role in the embryo transfer (ET) programs. The objective of this study was to evaluate the effects of Gonadotropin-releasing hormone (GnRH) or human chorionic gonadotropin (hCG) treatment on ovarian response in PMSG-superovulated ewes. Intravaginal pessaries containing 40 mg fluorogestone acetate (FGA) were inserted in all ewes (n=14) and remained in situ for 14 days. Two days prior to pessary removal, all ewes were treated with 1000 IU of PMSG. On the day of sponge removal (day 0), the females were randomly assigned to 3 treatments. The first group (T1; n=3) received no further treatment, while second group (T2; n=5) treated inter-muscular with GnRH (100 ug) at day 1, finally the third group (T3; n=6) treated inter-muscular with hCG (500 IU) during days 0-2. On day 8, laparotomy was performed to assess numbers of corpora lutea (CL) and anovulatory follicles (AF). Blood samples were collected for analysis of serum progesterone (P 4 ) using radioimmunoassay (RIA) method. The results obtained for first, second and third group were in number of CL (6.33±1.15, 9.00± 1.58 and 10.50± 5.54), number of AF (2.00 ± 3.46, 3.60 ± 6.50 and 4.16 ± 5.70), then the levels of P 4 (5.75 ± 4.45, 13.97 ± 4.67 and 13.22 ± 6.80 ng/ml), respectively. In conclusion GnRH or hCG treatment post sponge removal increases number of CL and improves luteal function after PMSG-superovulatory treatment. However, it didn’t reduce the number of AF.

Information

Item Type:	Journal
Divisions:	» Production animales, biotechnologie et sante » Institute of Agricultural and Veterinary Sciences
ePrint ID:	665
Date Deposited:	2017-02-08
Further Information:	Google Scholar
URI:	https://www.univ-soukahras.dz/en/publication/article/665

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