sameh MERADJI and Aboudihaj Barguigua (2016) Epidemiology and virulence of VIM-4 metallo-beta-lactamase-producing Pseudomonas aeruginosa isolated from burn patients in eastern Algeria. BURNS , volume 42(issue 4), 906-918, Elsevier
Scientific Publications
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Abstract
In
this
study,
we
investigated
the
prevalence
of
carbapenem-resistant
Pseudomonas
aerugi
nosa
(CRPA)
in
burn
patients
from
eastern
Algeria,
CRPA
virulence
factors
and
the
molecular
epidemiology
of
CRPA.
The
overall
prevalence
of
CRPA
was
48.38%.
Seven
(46.66%)
isolates
were
metallo-b-lactamases
(MBL)
producers
and
contained
the
MBL
genes
blaVIM-4 (n
=
6)
and
blaVIM-2 (n
=
1).
Risk
factors
for
CRPA
infection
were
urinary
catheter
use
and
intubation
(
p
=
0.008).
A
high
percentage
of
virulence
factors
(86.6%
of
these
isolates
were
able
to
produce
protease;
73.3%
of
isolates
has
DNase;
and
66.6%
were
haemolysin
positive)
was
observed
in
CRPA
isolates.
Among
the
seven
MBL-producing
isolates,
four
had
the
same
clonal
profile.
The
class
1
integrons,
which
contained
the
aadA7
gene
cassette,
were
detected
in
six
isolates.
The
16SrRNA
methylase
gene,
rmtB,
was
detected
in
one
strain.
All
CRPA
isolates
were
biofilm
formers.
A
study
on
the
kinetics
of
biofilm
production
revealed
that
biofilm
production
increased
when
the
concentration
of
imipenem
or
ciprofloxacin
and
the
incubation
time
increased.
This
is
the
first
study
to
report
the
presence
of
VIM-4-producing
P.
aeruginosa
from
North
Africa
and
also
of
the
high
prevalence
of
CRPA
isolates.
Based
on
our
study
of
burn
unit
patients,
the
high
percentage
of
P.
aeruginosa
with
virulence
factors
and
multi-drug
resistance
is
alarming.
this
study,
we
investigated
the
prevalence
of
carbapenem-resistant
Pseudomonas
aerugi
nosa
(CRPA)
in
burn
patients
from
eastern
Algeria,
CRPA
virulence
factors
and
the
molecular
epidemiology
of
CRPA.
The
overall
prevalence
of
CRPA
was
48.38%.
Seven
(46.66%)
isolates
were
metallo-b-lactamases
(MBL)
producers
and
contained
the
MBL
genes
blaVIM-4 (n
=
6)
and
blaVIM-2 (n
=
1).
Risk
factors
for
CRPA
infection
were
urinary
catheter
use
and
intubation
(
p
=
0.008).
A
high
percentage
of
virulence
factors
(86.6%
of
these
isolates
were
able
to
produce
protease;
73.3%
of
isolates
has
DNase;
and
66.6%
were
haemolysin
positive)
was
observed
in
CRPA
isolates.
Among
the
seven
MBL-producing
isolates,
four
had
the
same
clonal
profile.
The
class
1
integrons,
which
contained
the
aadA7
gene
cassette,
were
detected
in
six
isolates.
The
16SrRNA
methylase
gene,
rmtB,
was
detected
in
one
strain.
All
CRPA
isolates
were
biofilm
formers.
A
study
on
the
kinetics
of
biofilm
production
revealed
that
biofilm
production
increased
when
the
concentration
of
imipenem
or
ciprofloxacin
and
the
incubation
time
increased.
This
is
the
first
study
to
report
the
presence
of
VIM-4-producing
P.
aeruginosa
from
North
Africa
and
also
of
the
high
prevalence
of
CRPA
isolates.
Based
on
our
study
of
burn
unit
patients,
the
high
percentage
of
P.
aeruginosa
with
virulence
factors
and
multi-drug
resistance
is
alarming.
Information
| Item Type | Journal |
|---|---|
| Divisions |
» Faculty of Natural Sciences and Life |
| ePrint ID | 1826 |
| Date Deposited | 2019-06-22 |
| Further Information | Google Scholar |
| URI | https://univ-soukahras.dz/en/publication/article/1826 |
BibTex
@article{uniusa1826,
title={Epidemiology and virulence of VIM-4 metallo-beta-lactamase-producing Pseudomonas aeruginosa isolated from burn patients in eastern Algeria},
author={sameh MERADJI and Aboudihaj Barguigua},
journal={BURNS}
year={2016},
volume={volume 42},
number={issue 4},
pages={906-918},
publisher={Elsevier}
}
title={Epidemiology and virulence of VIM-4 metallo-beta-lactamase-producing Pseudomonas aeruginosa isolated from burn patients in eastern Algeria},
author={sameh MERADJI and Aboudihaj Barguigua},
journal={BURNS}
year={2016},
volume={volume 42},
number={issue 4},
pages={906-918},
publisher={Elsevier}
}