Abstract

In

this

study,

we

investigated

the

prevalence

of

carbapenem-resistant

Pseudomonas

aerugi
nosa

(CRPA)

in

burn

patients

from

eastern

Algeria,

CRPA

virulence

factors

and

the

molecular
epidemiology

of

CRPA.

The

overall

prevalence

of

CRPA

was

48.38%.

Seven

(46.66%)

isolates
were

metallo-b-lactamases

(MBL)

producers

and

contained

the

MBL

genes

blaVIM-4 (n

=

6)
and

blaVIM-2 (n

=

1).

Risk

factors

for

CRPA

infection

were

urinary

catheter

use

and

intubation
(

p

=

0.008).

A

high

percentage

of

virulence

factors

(86.6%

of

these

isolates

were

able

to
produce

protease;

73.3%

of

isolates

has

DNase;

and

66.6%

were

haemolysin

positive)

was
observed

in

CRPA

isolates.

Among

the

seven

MBL-producing

isolates,

four

had

the

same
clonal

profile.

The

class

1

integrons,

which

contained

the

aadA7

gene

cassette,

were

detected
in

six

isolates.

The

16SrRNA

methylase

gene,

rmtB,

was

detected

in

one

strain.

All

CRPA
isolates

were

biofilm

formers.

A

study

on

the

kinetics

of

biofilm

production

revealed

that
biofilm

production

increased

when

the

concentration

of

imipenem

or

ciprofloxacin

and

the
incubation

time

increased.

This

is

the

first

study

to

report

the

presence

of

VIM-4-producing
P.

aeruginosa

from

North

Africa

and

also

of

the

high

prevalence

of

CRPA

isolates.

Based

on

our
study

of

burn

unit

patients,

the

high

percentage

of

P.

aeruginosa

with

virulence

factors

and
multi-drug

resistance

is

alarming.

Information

Item Type:	Journal
Divisions:	» Faculty of Natural Sciences and Life
ePrint ID:	1826
Date Deposited:	2019-06-22
Further Information:	Google Scholar
URI:	https://www.univ-soukahras.dz/en/publication/article/1826

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