Abstract

ABSTRACT
In Algeria, the lack of information about antimicrobial resistance in commensal Escherichia coli of bovine intestinal microbiota, prompted us to conduct this study to characterize the E coli strains producing extended spectrum beta-lactamases.
A total of 300 strains of E coli are isolated from cattle faeces, the study was carried out in several localities of the governorates of Souk Ahras, Tébessa, Oum el Bouaghi and Constantine , all located in the East of Algeria
Antibiotic sensitivity is carried out by using disk diffusion method , with a panel of 13 antibiotics, colistin MIC is tested by broth microdilution method . High frequencies of resistance are recorded for ampicillin (63.67%), tetracycline (44.67%), cephalotin (26.33%) and trimethoprim / sulfamethoxazole (18.67%), however (1%) isolates are resistant to colistin. Co-resistance analysis reveals that (65 / 21.67%) isolates are resistant to more than three antibiotics and (89 / 29.67%) isolates are multidrug resistant (MDR). 77 different resistance phenotypes were isolated, more common are AMP (13.33%) and AMP-TE (11%).
Twelve different phenotypes produce extended spectrum ß-lactamases, all of which are MDR ranging from resistance to 5 antibiotics to the combination of 11.
Genotypic analysis by PCR of the 12 E coli isolates producing extended-spectrum beta-lactamases, reveals their belonging to the phylogenic groups A1 (83.33%) and B1 (16.66%), 03 strains harbor the group1 blaCTX-M gene and 12 harbor the blaTEM gene,
We report here the first detection of class 1 integrons and the qacE-sul1 sequence in 7 isolates, as well as the detection of the mcr-1 gene in 3 isolates and the qnrB and qnrS genes respectively in 3 and 2 isolates and this from cattle faeces in eastern Algeria.
Six isolates carry the tetA gene and 2 carry the tetB gene, the sul1 gene is detected in 9 isolates.
Twelve different pulsotypes are identified by PFGE. 10 virulence genes have been identified, with a predominance of the fimH and iutA genes, and a single isolate carries the stx1 gene. Three Virulotypes are classified as STEC(1 isolate), MNEC(2 isolates) and EAggEC( 3 isolates).
These high rates of antimicrobial resistance, as well as the multitude of antibiotic resistance genes and virulotypes detected, demonstrate that E. coli strains isolated from the bovine intestinal microbiota are a potential source of antibiotic resistance, emphasizes the urgent requirement to adopt measures to combat the phenomenon of antimicrobial resistance in order to control the spread of MDR bacteria from animals to humans and the environment.
Keywords: antimicrobial resistance, ESBL, genes, virulence factors, PCR, PFGE, cattle, eastern Algeria, Escherichia coli.

Information

Item Type:	Thesis
Divisions:	» Institute of Agricultural and Veterinary Sciences
ePrint ID:	2269
Date Deposited:	2020-08-17
Further Information:	Google Scholar
URI:	https://www.univ-soukahras.dz/en/publication/article/2269

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