(2020) Etude ethnobotanique, Caractérisation chimique et activités biologiques des huiles essentielles du genre Thymus de la région de Souk Ahras. Mohamed-Cherif Messaadia University - Souk Ahras
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Abstract
Thesis Title: Ethnobotanical study, Chemical characterization and biological activities of essential oils of the Thymus genus from the Souk Ahras region
Abstract
With the aim of enhancing the value of plants of the genus Thymus that are part of the natural heritage of the Souk Ahras region and to revitalize and preserve local empirical knowledge on these plants, we undertook an ethnobotanical study of 270 local population surveys followed by a series of chemical and biological analyses on six essential oils of three species of Thymus spp growing spontaneously in different regions of Souk Ahras.
The results of the survey show that older married women are the social group most interested in using Zaitra (VU=1.64 and CI=1.27), while adult married women are the group most familiarized with the different categories of use of the plant (ID≥3.91 and IE=0.70), Thyme is mainly used for therapeutic purposes (NFTher total = 88%) and particularly for the treatment of digestive disorders (NF= 70%). More than half of the users prefer to use the aerial part of the plant and prepare recipes in the form of infusions, most of which are taken orally.
The hydrodistillation of the aerial parts of the Thymus species gave yields of essential oils (EOs) between 0.9 and 2.35%. GC/MS chromatographic analyses of these EOs revealed the presence of 105 compounds, representing 95.70% to 99.46% of the total composition. Two chemotypes were determined: the thymol chemotype in the populations (TR1, TR2, TR3, TR4, TR6) and the α-pinene chemotype in the (TR7) population. Altitude and soil physico-chemical characteristics were the most influential factors in the variability of the yield and chemical composition of EOs.
The evaluation of the antibacterial activities of our EOs against six bacterial, strains four Gram (-) and two Gram (+) using the agar diffusion technique and the agar incorporation method, revealed that all of Thymus’s EOs studied, regardless of their species, provenance or chemical profile, exerted an interesting antibacterial activity on all of the Gram (+) and Gram (-) strains tested.
The antioxidant activities of Thymus EO evaluated using the DPPH test proved to be modest to low with IC50 between (513.77 and 2245.93 µg/ml), The most potent antiradical activity was noted in Thymus hirtus, from Boukabech (TR6) with (IC50 = 513.77 µg/ml), related to their thymol richness.
Keywords: Thymus, Souk Ahras, ethnobotanical study, hydrodistillation, essential oils, GC/MS chromatographic analyses, antibacterial activities, antioxidant activity.
Abstract
With the aim of enhancing the value of plants of the genus Thymus that are part of the natural heritage of the Souk Ahras region and to revitalize and preserve local empirical knowledge on these plants, we undertook an ethnobotanical study of 270 local population surveys followed by a series of chemical and biological analyses on six essential oils of three species of Thymus spp growing spontaneously in different regions of Souk Ahras.
The results of the survey show that older married women are the social group most interested in using Zaitra (VU=1.64 and CI=1.27), while adult married women are the group most familiarized with the different categories of use of the plant (ID≥3.91 and IE=0.70), Thyme is mainly used for therapeutic purposes (NFTher total = 88%) and particularly for the treatment of digestive disorders (NF= 70%). More than half of the users prefer to use the aerial part of the plant and prepare recipes in the form of infusions, most of which are taken orally.
The hydrodistillation of the aerial parts of the Thymus species gave yields of essential oils (EOs) between 0.9 and 2.35%. GC/MS chromatographic analyses of these EOs revealed the presence of 105 compounds, representing 95.70% to 99.46% of the total composition. Two chemotypes were determined: the thymol chemotype in the populations (TR1, TR2, TR3, TR4, TR6) and the α-pinene chemotype in the (TR7) population. Altitude and soil physico-chemical characteristics were the most influential factors in the variability of the yield and chemical composition of EOs.
The evaluation of the antibacterial activities of our EOs against six bacterial, strains four Gram (-) and two Gram (+) using the agar diffusion technique and the agar incorporation method, revealed that all of Thymus’s EOs studied, regardless of their species, provenance or chemical profile, exerted an interesting antibacterial activity on all of the Gram (+) and Gram (-) strains tested.
The antioxidant activities of Thymus EO evaluated using the DPPH test proved to be modest to low with IC50 between (513.77 and 2245.93 µg/ml), The most potent antiradical activity was noted in Thymus hirtus, from Boukabech (TR6) with (IC50 = 513.77 µg/ml), related to their thymol richness.
Keywords: Thymus, Souk Ahras, ethnobotanical study, hydrodistillation, essential oils, GC/MS chromatographic analyses, antibacterial activities, antioxidant activity.
Information
| Item Type | Thesis |
|---|---|
| Divisions | |
| ePrint ID | 2283 |
| Date Deposited | 2020-09-30 |
| Further Information | Google Scholar |
| URI | https://univ-soukahras.dz/en/publication/article/2283 |
BibTex
@phdthesis{uniusa2283,
title={Etude ethnobotanique, Caractérisation chimique et activités biologiques des huiles essentielles du genre Thymus de la région de Souk Ahras},
author={},
year={2020},
school={Mohamed-Cherif Messaadia University - Souk Ahras}
}
title={Etude ethnobotanique, Caractérisation chimique et activités biologiques des huiles essentielles du genre Thymus de la région de Souk Ahras},
author={},
year={2020},
school={Mohamed-Cherif Messaadia University - Souk Ahras}
}