Department of Biological Sciences

https://www.univ-soukahras.dz/en/dept/bio

Lecturer	Boudjemaa SAOUDI
Information	Bachelor - biochemistry Department of Biological Sciences Website : https://www.univ-soukahras.dz/en/module/2747 Semester : S5 Unit : UEF3.1.1 Credit : 6 Coefficient: 3
Content	Contents : I. General information II. ENZYME STRUCTURE AND PROPERTIES Structure and properties of enzymes - Monomeric enzymes (chymotrypsin) - Oligomeric enzymes - Isoenzymes (LDH) - Multienzyme complexes (FAS) III Protein-ligand interactions - Association on one site. - Association on n equivalent and independent sites. - Association of a ligand on two different sites. IV. Enzymatic kinetics - Michaelsian kinetics with one substrate (reminder) - Kinetics with two substrates - Multi-substrate kinetics V. Function and regulation of allosteric enzymes - Structural properties - Functional properties - Determination of kinetic constants from graphical representation (Hill...) VI. Mechanism of catalysis. - Topology and identification of active centres. - Functioning of coenzymes. - Activation of zymogens. - Specific markers of catalytic centres. - Mechanisms of action of serine proteases. - Mechanism of action of pyridoxal transferases. VII. Isolation and purification of enzymes - Origin of enzymes - Study methods VIII. Enzyme engineering - Nature and origin of enzymes VIII.1 - Enzyme immobilisation methods - Physical method: immobilisation by adsorption - Chemical method: immobilisation by covalent attachment to a support. - Immobilisation of enzymes and use in bioreactors VIII.2 - Applications of Enzymes in Biotechnology - Industrial preparation of enzymes - Production on an industrial scale - Applications IX. Tutorial work - The aim is to develop the ability to reason about enzymology problems and to learn to apply the concepts seen in class to interpret experimental data. experimental data. The tutorials will take the form of : - Exercises illustrating each chapter - Analysis of articles on the different points covered in class 3X. Practical work - Enzyme purification protocol: - Extraction, - Fractionation - Purification - Homogeneity criteria - Study of enzyme activities and the influence of certain physico-chemical parameters.
Evaluation	Assessment method : Assessment : Assessment of knowledge and weighting Assessment Weighting (%) Final exam 60 Tutorial 20 Practical work 20 Total 100 Tutorial 100% : 50% Micro-interrogations. 25. % Lectures. 15% Homework. 10% Other (Attendance and Participation) Practical work 100% : 60% Practical work reports. 40% Practical work examination.