Department of Biological Sciences

https://www.univ-soukahras.dz/en/dept/bio

Lecturer	Soumaya HABERRA
Information	Bachelor - biochemistry Department of Biological Sciences Website : https://www.univ-soukahras.dz/en/module/2751 Semester : S5 Unit : UEM3.1.1 Credit : 3 Coefficient: 2
Content	Contents : 1. Chromatographic methods 1.1 Definition and principle 1. 2. Parameters of a chromatographic analysis 1.3 Conditions for chromatographic separation 1.3.1 Low pressure chromatography 1.3.2 High pressure chromatography 1.4 The different types of chromatography and their applications 1. 4. 1 Liquid chromatography a. Partition chromatography b. Adsorption chromatography c. Ion exchange chromatography d. Gel permeation chromatography e. Hydrophobic interaction and hydroxyapatite chromatography f. Affinity chromatography 1. 4. 2 Gas chromatography (principle and applications) 2. Electrophoretic methods 2.1 Definition and principles 2.2 Parameters and conditions of use 2.2.1 Native electrophoresis 2.2.2 Electrophoresis in a dissociating and/or denaturing medium 2.3 Different types of electrophoresis and their applications 2.3.1 Zone electrophoresis 2. 3. 2 Electrophoresis on supports (agarose, cellulose acetate, polyacrylamide gel, etc.) polyacrylamide gel...) 2.3.3 Isoelectrofocalisation 2.3.4 Two-dimensional electrophoresis 2. 3. 5 Immunoelectrophoresis 3. Spectral methods 3.1 Molecular absorption spectrophotometry 3.1.1 Definitions and principles 3. 1. 2. Absorption spectra 3.1.3 Types and equipment 3.1.4 Applications 3.2 Atomic emission photometry (electron microscopy) 3.2.1 Definition and principle 3.2.2 Types and Equipment 3.2.3 Applications 3.3 Atomic absorption spectrophotometry 3.3.1 Definition and principle 3.3.2 Types and Equipment 3.3.3 Applications 3.4 Nuclear Magnetic Resonance 3.4.1 Definition and principle 3.4.2 Types and Equipment 3.4.3 Applications 4. Electron Microscopy 4.1 Transmission electron microscopy 4.1.1 Description of the apparatus 4. 1. 2. Principle of operation 4.1.3 Sample preparation 4.2 Scanning electron microscopy 4.2.2 Description of the device 4.2.3 How it works 4.2.4 Sample preparation 5. Immunological methods 5.1 Radioimmunology 5.2 Enzyme-linked immunosorbent assay (ELISA) 5. 3. immunofluorescence
Evaluation	Assessment: Assessment of knowledge and weighting Assessment comprises three parts: TD exam, TP report and final exam. The weighting of these tests is shown in the following table: Assessment Weighting (%) Final exam 60 Tutorial 20 Practical work (TP) 20 Total 100 Tutorial 100% : Control Weighting (%) Micro-interrogation (10/20) 50 Personal work 06/20 30 Oral presentation 04/20 20 Total 100 Practical work 100% : Assessment Weighting (%) Personal report of the 4 practical exercises (10/20) 50 Practical exam (06/20) 30 Attendance (04/20) 20 Total 100