Department of Biological Sciences

https://www.univ-soukahras.dz/en/dept/bio

Lecturer	Soumaya HABERRA
Information	Bachelor - Animal Biology and Physiology Department of Biological Sciences Website : https://www.univ-soukahras.dz/en/module/2777 Semester : S5 Unit : UEM3.1.1 Credit : 4 Coefficient: 2
Content	1. Chromatographic methods 1. 1. Definition and principle 1. 2. Parameters of a chromatographic analysis 1. 3. Conditions for separation by chromatography 1. 3. 1. Low pressure chromatography 1. 3. 2. High pressure chromatography 1. 4. The different types of chromatography and their applications 1. 4. 1. Liquid chromatography has. Partition Chromatography b. Adsorption chromatography vs. Ion exchange chromatography d. Gel permeation chromatography e. Chromatography of hydrophobic and hydroxyapatite interactions f. Affinity chromatography 1. 4. 2. Gas chromatography (principle and applications) 2. Electrophoretic methods 2. 1. Definition and principles 2. 2. Parameters and conditions of realization 2. 2. 1. Native electrophoresis 2. 2. 2. Electrophoresis in a dissociating and/or denaturing medium 2. 3. Different types of electrophoresis and their applications 2. 3. 1. Zone electrophoresis 2. 3. 2. Electrophoresis on supports (agarose, cellulose acetate, gel polyacrylamide...) 2. 3. 3. Isoelectrofocusing 2. 3. 4. Two-dimensional electrophoresis 2. 3. 5. Immunoelectrophoresis 3. Spectral methods 3. 1. Molecular absorption spectrophotometry 3. 1. 1. Definitions and principles 3. 1. 2. Absorption spectrum 3. 1. 3. Types and equipment 3. 1. 4. Applications 3. 2. Atomic emission photometry (electron microscopy) 3. 2. 1. Definition and principle 3. 2. 2. Types and Devices 3. 2. 3. Applications 3. 3. Atomic absorption spectrophotometry 3. 3. 1. Definition and principle 3. 3. 2. Types and Equipment 3. 3. 3. Applications 3. 4. Nuclear magnetic resonance 3. 4. 1. Definition and principle 3. 4. 2. Types and Equipment 3. 4. 3. Applications 4. Electron microscopy 4. 1. Transmission electron microscopy 4. 1. 1. Description of the device 4. 1. 2. Operating principle 4. 1. 3. Sample preparation 4. 2. Scanning electron microscopy 4. 2. 2. Description of the device 4. 2. 3. Operating principle 4. 2. 4. Sample preparation 5. Immunological methods 5. 1. Radioimmunology 5. 2. Enzyme-linked immunosorbent assay (ELISA) 5. 3. Immunofluorescence
Evaluation	Final Exam 60%+ directed work 20%+ pratical 20% Directed Work 100%: 30% Personal work (6/20) 50% Micro-questions (10/20) 20% Oral presentation (4/20) Practical work 100%: 50% Personal report, Reports of the 4 practicals (10/20) 30% TP exam (6/20) 20% Participation (4/20)