Department of Biological Sciences

https://www.univ-soukahras.dz/en/dept/bio

Lecturer	Soumaya HABERRA
Information	Bachelor - Biology and ecology of aquatic environments Department of Biological Sciences Website : https://www.univ-soukahras.dz/en/module/3246 Semester : S5 Unit : UEM 3.1.1 Credit : 4 Coefficient: 2
Content	C1. CHROMATOGRAPHIC METHODS 1.1 Definition and principle 1. 2. Chromatographic analysis parameters 1.3 Chromatographic separation conditions 1.3.1 Low-pressure chromatography 1.3.2 High-pressure chromatography 1.4 Different types of chromatography and their applications 1.4.1 Liquid chromatography a. Partition chromatography b. Adsorption chromatography c. Ion exchange chromatography d. Gel permeation chromatography e. Hydrophobic interaction and hydroxyapatite chromatography f. Affinity chromatography 1. 4. 2 Gas chromatography (principle and applications) 2. ELECTROPHORETIC METHODS 2.1 Definition and principles 2.2 Parameters and conditions of use 2.2.1 Native electrophoresis 2.2.2 Electrophoresis in dissociating and/or denaturing media 2.3 Different types of electrophoresis and their applications 2.3.1 Zone electrophoresis 2. 3. 2 Electrophoresis on supports (agarose, cellulose acetate, polyacrylamide gel, etc.) polyacrylamide gel...) 2. 3. 3 Isoelectrofocusing 2.3.4 Two-dimensional electrophoresis 2. 33. SPECTRAL METHODS 3.1 Molecular absorption spectrophotometry 3.1.1 Definitions and principles 3. 1. 2. Absorption spectra 3.1.3 Types and equipment 3.1.4 Applications 3.2 Atomic emission photometry (electron microscopy) 3.2.1 Definition and principle 3.2.2 Types and equipment 3.2.3 Applications 3.3 Atomic absorption spectrophotometry 3.3.1 Definition and principle 3.3.2 Types and equipment 3.3.3 Applications 3.4 Nuclear Magnetic Resonance 3.4.1 Definition and principle 3.4.2 Types and equipment 3. 4. 3 Applications 4. ELECTRON MICROSCOPY 4.1 Transmission electron microscopy 4.1.1 Device description 4. 1. 2. Principle of operation 4.1.3 Sample preparation 4.2 Scanning electron microscopy 4.2.2 Device description 4.2.3 Principle of operation 4. 2. 4 Sample preparation 5. IMMUNOLOGICAL METHODS 5.1 Radioimmunology 5.2 Enzyme Immunoassay
Evaluation	Final exam: 60% of the total Tutorial: 20% Practical work: 20% Tutorial 100% Micro-interogation 50% Personnal work 30% Oral presentation: 20% Practical work Practical work 100% Personal report on the 4 practical sessions 50% Practical exam 30% Participation 20%