Department of Ecology and Environment

https://www.univ-soukahras.dz/en/dept/eco-env

Lecturer	Nesrine GHERAIRIA
Information	Master - Fundamental and Applied Ecology Department of Ecology and Environment Website : https://www.univ-soukahras.dz/en/module/3307 Semester : S3 Unit : UEM1 Credit : 6 Coefficient: 3
Content	ubject Content: A. Biochemical Analysis Tools: I. Review of Units Used in Biological Analysis II. Sampling, Sample Preparation, and Storage III. Centrifugation, Ultracentrifugation (Cellular and Subcellular Fractionation) IV. Cytological and Cyto-Histochemical Techniques Immunological Techniques Flow Cytometry V. Electrophoretic Techniques Electrophoresis on Cellulose Acetate Electrophoresis on Polyacrylamide Gel (PAGE, SDS-PAGE) Agarose Gel Electrophoresis VI. Chromatographic Techniques Adsorption and Partition Chromatography (Paper and Thin-Layer Chromatography: TLC) Gel Filtration Chromatography Ion Exchange Chromatography High-Performance Liquid Chromatography (HPLC) Gas Chromatography (GC) Spectroscopic Methods: Atomic Absorption Spectrometry (UV-Vis) Atomic Emission Spectrometry Mass Spectrometry (MS) B. Molecular Analysis Tools: I. Nucleic Acids II. Extraction and Purification of Nucleic Acids III. Polymerase Chain Reaction (PCR, rt-PCR) IV. PCR Applications V. Gene Amplification VI. Molecular Species Identification VII. Phylogenetic Tree Determination VIII. Cloning IX. Gene Hybridization and Expression.\"
Evaluation	Assessment Weighting (%) Final Exam 50 Tutorials Practical Work 50 Practical Work 100%: 80% Lab Report. 15% Lab Exam. 5% Other.