Department of Biological Sciences

Lecturer	Nadjette BOURAFA
Information	Bachelor - Microbiology Department of Biological Sciences Website : https://www.univ-soukahras.dz/en/module/4473 Semester : S5 Unit : UEF 3.1.2.1 Credit : 6 Coefficient: 3
Content	Part I: Molecular biology: 1. Expression of genetic information: protein synthesis (Transcription, Translation). 2. Regulation of gene expression: Transcriptional regulation, Regulation translational. 3. Basic techniques of molecular biology: - preparation of nucleic acids (extraction and purification) - separations of nucleic acids (electrophoresis on agarose gel, in the field pulsed,…...). - detection, characterization and identification of nucleic acids (transfer to membrane, marking, hybridization, etc.). - DNA sequencing. - in vitro amplification of nucleic acids (PCR, RT (reverse-transcriptase)-PCR …). Part II: genetic engineering: 1. in vivo cloning: 1.1. Elements necessary for cloning: DNA to be cloned, restriction enzymes, ligation enzymes, cloning vectors, their construction and their characteristics, host cells. 1.2. The stages of cloning: construction of the vector, insertion of the DNA to be cloned, transformation of bacteria, selection of recombinants, analysis of recombinants. 2. Recombinant DNA technology: Synthesis of recombinant proteins, cDNA and expression vectors. Example of protein production by E. coli and by Saccharomyces cerevisiae.
Evaluation	60% EXAMS 40% TUTORIALS 15% TETS 42.5TSTS 42.5ùPARTICIPATION